STX17 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

STX17 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(56)

Rezensionen (1)

Kat-Nr. : 17815-1-AP

Synonyme

Syntaxin 17, Syntaxin-17

---

• 

  WB analysis of HepG2 using 17815-1-AP

  WB result of STX17 antibody (17815-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STX17 transfected HepG2 cells.

• 

  WB analysis using 17815-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 17815-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 17815-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 17815-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17815-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17815-1-AP

  IP result of anti-STX17 (IP:17815-1-AP, 4ug; Detection:17815-1-AP 1:1000) with HepG2 cells lysate 3600ug.

• 

  IHC staining of mouse testis using 17815-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17815-1-AP (STX17 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17815-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17815-1-AP (STX17 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17815-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17815-1-AP (STX17 antibody at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A375-Zellen, humanes Hodengewebe, Jurkat-Zellen, L02-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 43 publications below
• IHC: See 4 publications below
• IF: See 16 publications below
• IP: See 6 publications below
• CoIP: See 4 publications below

Produktinformation

17815-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA STX17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STX17 fusion protein Ag12134
• Vollständiger Name: syntaxin 17
• Berechnetes Molekulargewicht: 302 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33-38 kDa
• GenBank-Zugangsnummer: BC111009
• Gene symbol: STX17
• Gene ID (NCBI): 55014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for the fusion of cellular membranes. Syntaxin 17 (STX17) is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC, and the Golgi.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Cancer cells escape autophagy inhibition via NRF2-induced macropinocytosis.; Authors - Hua Su
human	WB,IF,IP	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB,IF	Autophagy; Atractylenolide I inhibits angiogenesis and reverses sunitinib resistance in clear cell renal cell carcinoma through ATP6V0D2-mediated autophagic degradation of EPAS1/HIF2α; Authors - Qinyu Li
human	WB	Autophagy; DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.; Authors - Xiaoyu Tian
mouse	WB	Autophagy; PMID: 32432943; Authors - Zu-Guo Zheng ; KD Validated
human	WB	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Jane (Verified Customer) (01-07-2022)

No working, non specific bands.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Heart/Cardiomyocytes

STX17 Antibody Western Blot,Immunohistochemistry validation (1:500 dilution) in Heart/Cardiomyocytes (Cat no:17815-1-AP)