Syntaxin 1B Rekombinanter Antikörper

Syntaxin 1B Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240156B9

Kat-Nr. : 83298-1-PBS

Synonyme

Syntaxin 1B, STX1B, Syntaxin 1A, 240156B9, STX1B1

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  WB analysis of Y79 using 83298-1-RR (same clone as 83298-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 83298-1-RR (STX1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00312-1

  Sandwich ELISA standard curve of MP00312-1, Human STX1B Monoclonal Matched Antibody Pair - PBS only. 83298-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10275. 83298-4-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

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  Sample test of MP00312-1

  The mean human STX1B concentration was determined to be 7.2 μg/mL in rat Brain tissue lysate based on a 3.0 mg/mL extract load. The mean human STX1B concentration was determined to be 18.1 μg/mL in mouse Brain tissue lysate based on a 2 mg/mL extract load. The mean human STX1B concentration was determined to be 7.0 ng/mL in rat kidney tissue lysate based on a 3 mg/mL extract load. The mean human STX1B concentration was determined to be 15.1 ng/mL in mouse kidney tissue lysate based on a 1.5 mg/mL extract load.

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  IHC staining of mouse eye using 83298-1-RR (same clone as 83298-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 83298-1-RR (same clone as 83298-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STX1B antibody (83298-1-RR, Clone: 240156B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 83298-1-RR (same clone as 83298-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STX1B antibody (83298-1-RR, Clone: 240156B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 83298-1-RR (same clone as 83298-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Stx1b Recombinant Antibody (83298-1-RR, Clone:240156B9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83298-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00312-1

  Cytometric bead array standard curve of MP00312-1, STX1B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83298-1-PBS. Detection antibody: 83298-4-PBS. Standard: Ag10275. Range: 1.25-40 ng/mL.

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  Affinity and Kinetic Characterization of 83298-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 83298-1-PBS against Human STX1B were performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 83298-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83298-1-RR against Human STX1B were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83298-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Syntaxin 1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Syntaxin 1B fusion protein Ag10275
• Vollständiger Name: syntaxin 1B
• Berechnetes Molekulargewicht: 288 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33-35 kDa
• GenBank-Zugangsnummer: BC062298
• Gene symbol: STX1B
• Gene ID (NCBI): 112755
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641).