Syntaxin 3 Polyklonaler Antikörper

Syntaxin 3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 15556-1-AP

Synonyme

STX3, STX3A, Syntaxin-3

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  WB analysis of SH-SY5Y using 15556-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 15556-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 15556-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 15556-1-AP

  IP result of anti-Syntaxin 3 (IP:15556-1-AP, 4ug; Detection:15556-1-AP 1:1000) with SH-SY5Y cells lysate 3600ug.

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  IHC staining of human kidney using 15556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15556-1-AP (Syntaxin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, humanes Plasmagewebe, Neuro-2a-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 8 publications below
• IP: See 3 publications below

Produktinformation

15556-1-AP bindet in WB, IHC, IF, IP, ELISA Syntaxin 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Syntaxin 3 fusion protein Ag7925
• Vollständiger Name: syntaxin 3
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC007405
• Gene symbol: STX3
• Gene ID (NCBI): 6809
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxin-3 (also known as STX3) is a member of the syntaxin. Syntaxin-3 is a significant apical targeting protein in the epithelial membrane and in exocytosis process; it also acts as a vesicle transporter by cellular receptor in neutrophils, which is crucial for protein trafficking event (PMID: 25630102). It has been reported that syntaxin-3 has an important role in the growth of neurites, and serves as a direct target for omega-6 arachidonic acid (PMID: 1659826).

Publikationen

Species	Application	Title
mouse	WB,IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
human	WB,IP	Mol Ther; circMRPS35 promotes malignant progression and cisplatin resistance in hepatocellular carcinoma; Authors - Peng Li
mouse	IF	PLoS Genet; BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.; Authors - Ying Hsu
mouse	IHC	Hum Mol Genet; Photoreceptor cilia, in contrast to primary cilia, grant entry to a partially assembled BBSome.; Authors - Ying Hsu
mouse	IF	Front Mol Neurosci; Farnesylation of the Transducin G Protein Gamma Subunit Is a Prerequisite for Its Ciliary Targeting in Rod Photoreceptors.; Authors - Celine Brooks
human	WB	J Biol Chem; Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.; Authors - Wulin Aerbajinai