Syntaxin 4 Polyklonaler Antikörper

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• KD/KO Validated

Syntaxin 4 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 14988-1-AP

Synonyme

Syntaxin-4, STX4A, STX4, Renal carcinoma antigen NY-REN-31

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  WB analysis using 14988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14988-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14988-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 14988-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 14988-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 14988-1-AP

  IP result of anti-Syntaxin 4 (IP:14988-1-AP, 4ug; Detection:14988-1-AP 1:1500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human stomach using 14988-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, Jurkat-Zellen, Rattennierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below

Produktinformation

14988-1-AP bindet in WB, IHC, IP, ELISA Syntaxin 4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Syntaxin 4 fusion protein Ag6973
• Vollständiger Name: syntaxin 4
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: BC002436
• Gene symbol: STX4
• Gene ID (NCBI): 6810
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Brain; Reduced oligodendrocyte exosome secretion in multiple system atrophy involves SNARE dysfunction.; Authors - Zhenwei Yu
human	WB	Front Immunol; Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing.; Authors - Jamie A Lopez
human	WB	Int J Biol Macromol; PKCε controls the fusion of secretory vesicles in mast cells in a phosphatidic acid-dependent mode.; Authors - Emilio M Serrano-López
human	WB	Br J Pharmacol; CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.; Authors - Fang Bian
human	WB	J Biol Chem; Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.; Authors - Wulin Aerbajinai
human	WB	J Transl Med; Angiopoietin2-mediated caveolin1 phosphorylation regulating transcytosis of renal tubular epithelial cell contributes to the occurrence of albuminuria under high glucose exposure.; Authors - Jing Liu