STX5 Polyklonaler Antikörper

STX5 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (2)

Kat-Nr. : 26711-1-AP

Synonyme

SED5, STX5, STX5A, syntaxin 5

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  WB analysis using 26711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26711-1-AP (STX5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 26711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 26711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 26711-1-AP (STX5 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 26711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 26711-1-AP (STX5 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

26711-1-AP bindet in WB, IHC, IF-P, ELISA STX5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STX5 fusion protein Ag25098
• Vollständiger Name: syntaxin 5
• Berechnetes Molekulargewicht: 32 kDa, 34 kDa, 42 kDa
• Beobachtetes Molekulargewicht: 35 and 42 kDa
• GenBank-Zugangsnummer: BC012137
• Gene symbol: STX5
• Gene ID (NCBI): 6811
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (01-31-2025)

very good for WB

• Applications: Western Blot

FH

Wei (Verified Customer) (06-28-2020)

Double band for whole heart tissues. The upper band is much dense than the lower band.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart