Syntaxin 6 Rekombinanter Antikörper

Syntaxin 6 Rekombinant Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

242100A9

Kat-Nr. : 84627-5-RR

Synonyme

Syntaxin-6, STX6, 242100A9

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  WB analysis using 84627-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84627-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 84627-5-RR

  IP result of anti-Syntaxin 6 (IP:84627-5-RR, 4ug; Detection:84627-5-RR 1:1500) with mouse brain tissue lysate 1840 ug.

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  IHC staining of mouse brain using 84627-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 84627-5-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (84627-5-RR, Clone: 242100A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84627-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84627-5-RR against Human Syntaxin 6 were performed. The affinity constant is 1.55 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, fetales humanes Hirngewebe, Maushirngewebe, Neuro-2a-Zellen, PC-12-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84627-5-RR bindet in WB, IHC, IF/ICC, IP, ELISA Syntaxin 6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Syntaxin 6 fusion protein Ag1284
• Vollständiger Name: syntaxin 6
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC009944
• Gene symbol: Syntaxin 6
• Gene ID (NCBI): 10228
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.