Syntaxin 8 Polyklonaler Antikörper

Syntaxin 8 Polyklonal Antikörper für FC, IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12206-1-AP

Synonyme

CARB, STX8, syntaxin 8

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Galerie der Validierungsdaten

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  WB analysis of human heart using 12206-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12206-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12206-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12206-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12206-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12206-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12206-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12206-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12206-1-AP (Syntaxin 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 12206-1-AP

  IP Result of anti-Syntaxin 8 (IP:12206-1-AP, 4ug; Detection:12206-1-AP 1:500) with A375 cells lysate 3600ug.

• 

  IF Staining of HepG2 using 12206-1-AP

  Immunofluorescent analysis of HepG2 cells, using STX8 antibody 12206-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 12206-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Syntaxin 8 antibody (12206-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, A375-Zellen, humanes Hirngewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, Maus-Pankreasgewebe
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12206-1-AP bindet in WB, IP, IF, FC, CoIP, ELISA Syntaxin 8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Syntaxin 8 fusion protein Ag2850
• Vollständiger Name: syntaxin 8
• Berechnetes Molekulargewicht: 236 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC009713
• Gene symbol: STX8
• Gene ID (NCBI): 9482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxin 8 (STX8), also known as CIP-1-associated regulator of cyclin B (CARB ), is a protein belongs to the syntaxin family. The gene of STX8 maps to chromosomal band 17p12 and it encodes a 236-amino-acid protein containing 1 t-SNARE coiled-coil homology domain. STX8 mRNA is broadly expressed in multiple human tissues, including heart, brain, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas (PMID: 10198254). STX8 is involved in protein trafficking from early to late endosomes via vesicle fusion and exocytosis.

Publikationen

Species	Application	Title
rat	WB,CoIP	J Cell Biol; VAMP4 regulates insulin levels by targeting secretory granules to lysosomes; Authors - Min Li ; KD Validated
mouse		PLoS One; The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification.; Authors - Mona Abed