Syntaxin 8 Rekombinanter Antikörper

Syntaxin 8 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251516G10

Kat-Nr. : 86580-1-PBS

Synonyme

STX8, Syntaxin-8

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  WB analysis using 86580-1-RR (same clone as 86580-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86580-1-RR (STX8 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86580-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP02538-1

  The mean Syntaxin 8 concentration was determined to be 28.1 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 43.4 ng/mL in A375 cell extract based on a 1.6 mg/mL extract load.

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  Cytometric bead array standard curve of MP02538-1

  Cytometric bead array standard curve of MP02538-1, Syntaxin 8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86580-2-PBS. Detection antibody: 86580-1-PBS. Standard: Ag2850. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 86580-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86580-1-RR against Human Syntaxin 8 were performed. The affinity constant is 0.454 nM.

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Geprüfte Anwendungen

Produktinformation

86580-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA Syntaxin 8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Syntaxin 8 fusion protein Ag2850
• Vollständiger Name: syntaxin 8
• Berechnetes Molekulargewicht: 236 aa, 27 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC009713
• Gene symbol: Syntaxin 8
• Gene ID (NCBI): 9482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxin 8 (STX8), also known as CIP-1-associated regulator of cyclin B (CARB ), is a protein belonging to the syntaxin family. The gene of STX8 maps to chromosomal band 17p12 and it encodes a 236-amino-acid protein containing 1 t-SNARE coiled-coil homology domain. STX8 mRNA is broadly expressed in multiple human tissues, including the heart, brain, kidney, liver, lung, placenta, skeletal muscle, spleen, and pancreas (PMID: 10198254). STX8 is involved in protein trafficking from early to late endosomes via vesicle fusion and exocytosis.