STXBP1 Polyklonaler Antikörper

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• KD/KO Validated

STXBP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 11459-1-AP

Synonyme

N-Sec1, N Sec1, Munc18-1, MUNC18 1, FLJ37475

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  WB analysis using 11459-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11459-1-AP

  WB result of STXBP1 antibody (11459-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STXBP1 transfected HeLa cells.

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  WB analysis of Y79 using 11459-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11459-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11459-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11459-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11459-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11459-1-AP

  IP result of anti-STXBP1 (IP:11459-1-AP, 4ug; Detection:11459-1-AP 1:1000) with HeLa cells lysate 2400ug.

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  IHC staining of human brain using 11459-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11459-1-AP (STXBP1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 11459-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11459-1-AP (STXBP1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A375 using 11459-1-AP

  Immunofluorescent analysis of A375 cells using 11459-1-AP (STXBP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, A549-Zellen, humanes Hirngewebe, Jurkat-Zellen, SH-SY5Y-Zellen, Y79-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

11459-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA STXBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STXBP1 fusion protein Ag1978
• Vollständiger Name: syntaxin binding protein 1
• Berechnetes Molekulargewicht: 594 aa, 68 kDa
• Beobachtetes Molekulargewicht: 65-68 kDa
• GenBank-Zugangsnummer: BC015749
• Gene symbol: STXBP1
• Gene ID (NCBI): 6812
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB, IHC	EMBO J; Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly.; Authors - Xianping Wang
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	WB	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta
rat	WB	Neuroscience; SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.; Authors - Xing-Lian Duan
mouse	WB, IHC	PLoS One; Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance.; Authors - Poppy Datta
mouse	WB	Cell Rep; Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies; Authors - Nadeem Murtaza