STXBP5 Polyklonaler Antikörper

STXBP5 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24512-1-AP

Synonyme

Lethal(2) giant larvae protein homolog 3, LLGL3, Syntaxin-binding protein 5, Tomosyn-1

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  WB analysis using 24512-1-AP

  Mouse brain tissue was subjected to SDS PAGE, followed by western blot with 24512-1-AP (STXBP5 antibody) at a dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 24512-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24512-1-AP (STXBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 24512-1-AP

  IP result of anti-STXBP5 (IP:24512-1-AP, 5ug; Detection:24512-1-AP 1:300) with rat brain tissue lysate 4000ug.

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  IF Staining of SH-SY5Y using 24512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STXBP5 antibody (24512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HUVEC using 24512-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using STXBP5 antibody (24512-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen, HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

24512-1-AP bindet in WB, IF/ICC, IP, ELISA STXBP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STXBP5 fusion protein Ag21629
• Vollständiger Name: syntaxin binding protein 5 (tomosyn)
• Berechnetes Molekulargewicht: 1151 aa, 128 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC113382
• Gene symbol: STXBP5
• Gene ID (NCBI): 134957
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxin-binding protein 5 (STXBP5, also known as tomosyn) is a cytosolic syntaxin-binding protein that can displace MUNC18 from syntaxin-1. It is a soluble R-SNARE protein that sequesters target SNAREs through the C-terminal VAMP-like domain. STXBP5 plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release.

Publikationen

Species	Application	Title
human	WB	Sci Rep; The syntaxin-binding protein STXBP5 regulates progerin expression; Authors - Hongqian Qi ; KD Validated