SULT1E1 Polyklonaler Antikörper

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• KD/KO Validated

SULT1E1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12522-1-PBS

Synonyme

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  WB analysis of human lung using 12522-1-AP (same clone as 12522-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 12522-1-AP (same clone as 12522-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human adrenal gland using 12522-1-AP (same clone as 12522-1-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 12522-1-AP (same clone as 12522-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 12522-1-AP (same clone as 12522-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 12522-1-AP (same clone as 12522-1-PBS)

  IP result of anti-SULT1E1 (IP:12522-1-AP, 4ug; Detection:12522-1-AP 1:400) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12522-1-AP (same clone as 12522-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12522-1-AP (same clone as 12522-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12522-1-PBS bindet in WB, IHC, IP, Indirect ELISA SULT1E1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SULT1E1 fusion protein Ag3216
• Vollständiger Name: sulfotransferase family 1E, estrogen-preferring, member 1
• Berechnetes Molekulargewicht: 294 aa, 35 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC027956
• Gene symbol: SULT1E1
• Gene ID (NCBI): 6783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SULT1E1, also known as estrogen sulfotransferase, catalyzes the sulfation of endogenous estrogens as well as xenobiotic estrogen-like chemicals, converting them into the inactive form. SULT1E1 is a 33-35 kDa cytosolic protein that has been detected in human hepatic and nonhepatic tissues (17035602). SULT1E1 is expressed in breast and endometrial tissues, and some studies have investigated its implication in tumor development. Higher expression of SULT1E1 was observed in breast cancer tissues compared with normal breast tissues (22380844). This antibody detected a major band around 35 kD in lysates of mouse kidney, human liver and so on.