SUMO1 Polyklonaler Antikörper

SUMO1 Polyklonal Antikörper für FC, IF, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (1)

Kat-Nr. : 10329-1-AP

Synonyme

DAP 1, GAP modifying protein 1, GMP1, OFC10, PIC1, Sentrin, SMT3, SMT3 homolog 3, SMT3C, SMT3H3, SUMO 1, SUMO1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1, UBL1

---

Galerie der Validierungsdaten

• 

  WB analysis using 10329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10329-1-AP (SUMO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10329-1-AP

  IP result of anti-SUMO1(IP:10329-1-AP, 4ug; Detection:10329-1-AP 1:1000) with HeLa cells lysate 1200 ug.

• 

  IHC staining of human testis using 10329-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10329-1-AP (SUMO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10329-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10329-1-AP (SUMO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10329-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SUMO1 antibody (10329-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 10329-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SUMO1 antibody (10329-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A549 using 10329-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human SUMO1 (10329-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

---

Geprüfte Anwendungen

• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, A549-Zellen
• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

10329-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA SUMO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUMO1 fusion protein Ag0414
• Vollständiger Name: SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 10-12 kDa, 80-90 kDa
• GenBank-Zugangsnummer: BC006462
• Gene symbol: SUMO1
• Gene ID (NCBI): 7341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Small ubiquitin-related modifier 1 (SUMO1) belongs to the ubiquitin family and the SUMO subfamily as it contains 1 ubiquitin-like domain and is also 18% identical to ubiquitin (PMID: 9654451).

1. What is the molecular weight of SUMO1?

The molecular weight of SUMO1 is 12 kDa.

2. What is the cellular localization of SUMO1?

SUMO1 is located in the nuclear membrane and is recruited by BCL11A to the nuclear body (PMID: 18681895).

3. What modifications is SUMO1 subjected to?

SUMO1 function occurs after the cleavage of its precursor form by SENP1 or SENP2 (PMID: 27576863). Polymeric SUMO1 chains also undergo polyubiquitination by RNF4 (PMID: 18408734).

4. What is the function of SUMO1?

SUMO1 post-translationally modifies many proteins that have roles in an array of processes including transcriptional regulation, chromatin structure, and DNA repair. SUMO1 can covalently attach itself to protein as a monomer or a lysine-linked polymer via an isopeptide bond (PMID:11124955). This SUMO modification can regulate the localization and activity of the affected proteins.

5. What is SUMO1's involvement in disease?

Defects in SUMO1 as a result of a chromosomal translocation are associated with non-syndromic orofacial cleft type 10, or non-syndromic cleft lip, and are associated with cleft palate in two-thirds of cases (PMID: 18983974).

Publikationen

Species	Application	Title
human	WB	Sci Adv; XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility; Authors - Ming Kuang
human	WB	Theranostics; Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.; Authors - Shuai Huang
human	WB	Anal Chem; Sensitive detection of intracellular sumoylation via SNAP tag-mediated translation and RNA polymerase-based amplification.; Authors - Yong Yang
human	IP	mSystems; Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.; Authors - Xiaoqing Liu
human	WB	J Mol Cell Biol; SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.; Authors - Xiaoli Xu
mouse	IP	Int J Mol Med; SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary.; Authors - Jing Cao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Zoe (Verified Customer) (10-08-2021)

Hadn’t previously had much luck with antibodies on my Tricky construct so was great to use this one!

• Applications: Western Blot

Verwandte Produkte

P53 Polyclonal antibody

10442-1-AP

MDM2 Polyclonal antibody

19058-1-AP

RANGAP1 Polyclonal antibody

11102-1-AP

UBA2 Polyclonal antibody

15347-1-AP

SENP1 Polyclonal antibody

25349-1-AP