SUMO1 Monoklonaler Antikörper

SUMO1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B5B5

Kat-Nr. : 67559-1-PBS

Synonyme

3B5B5, GAP-modifying protein 1, GMP1, OK/SW-cl.43, Sentrin

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  WB analysis using 67559-1-Ig (same clone as 67559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67559-1-Ig (SUMO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 67559-1-Ig (same clone as 67559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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  IHC staining of rat small intestine using 67559-1-Ig (same clone as 67559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67559-1-Ig (same clone as 67559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67559-1-Ig (SUMO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67559-1-Ig (same clone as 67559-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SUMO1 antibody (67559-1-Ig, Clone: 3B5B5 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67559-1-Ig (same clone as 67559-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SUMO1 antibody (67559-1-Ig, Clone: 3B5B5 ) at dilution of 1:3200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67559-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67559-1-PBS bindet in WB, IHC, IF/ICC, ELISA SUMO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SUMO1 fusion protein Ag29402
• Vollständiger Name: SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 15-100 kDa
• GenBank-Zugangsnummer: BC006462
• Gene symbol: SUMO1
• Gene ID (NCBI): 7341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.This antibody can detect endogenous levels of SUMOylated proteins (e.g. SUMO-1-RanGAP at 80-90 kD).