SUMO2/3 Monoklonaler Antikörper

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• KD/KO Validated

SUMO2/3 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1B3

Kat-Nr. : 67154-1-PBS

Synonyme

SUMO2, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2, Sentrin 2

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  WB analysis of HEK-293 using 67154-1-Ig (same clone as 67154-1-PBS)

  WB result of SUMO2/3 antibody (67154-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUMO2/3 transfected HEK-293 cells. This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

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  WB analysis using 67154-1-Ig (same clone as 67154-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67154-1-Ig (same clone as 67154-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67154-1-Ig (same clone as 67154-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67154-1-Ig (same clone as 67154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67154-1-Ig (same clone as 67154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67154-1-Ig (same clone as 67154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67154-1-Ig (same clone as 67154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67154-1-Ig (SUMO2/3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67154-1-Ig (same clone as 67154-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (67154-1-Ig, Clone: 1A1B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67154-1-Ig (same clone as 67154-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUMO2/3 antibody (67154-1-Ig, Clone: 1A1B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67154-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67154-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SUMO2/3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SUMO2/3 fusion protein Ag28672
• Vollständiger Name: SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC008450
• Gene symbol: SUMO2
• Gene ID (NCBI): 6613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.