SUMO2/3 Rekombinanter Antikörper

SUMO2/3 Rekombinant Antikörper für IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241156E1

Kat-Nr. : 84017-5-PBS

Synonyme

SUMO3, SUMO 2, SMT3H1, SMT3A, SMT3 homolog 1

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  IF Staining of HepG2 using 84017-5-RR (same clone as 84017-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUMO3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84017-5-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84017-5-RR (same clone as 84017-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SUMO3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84017-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84017-5-RR (same clone as 84017-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUMO2/3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84017-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84017-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84017-5-RR against Human SUMO2/3 were performed. The affinity constant is 6.81 nM.

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Geprüfte Anwendungen

Produktinformation

84017-5-PBS bindet in IF/ICC, Indirect ELISA SUMO2/3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SUMO2/3 fusion protein Ag1135
• Vollständiger Name: SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
• Berechnetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC008420
• Gene symbol: SUMO3
• Gene ID (NCBI): 6612
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.