SUNC1 Polyklonaler Antikörper

SUNC1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 26434-1-AP

Synonyme

SUN3, SUN domain-containing protein 3, Sad1/unc-84 domain-containing protein 1

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  WB analysis of mouse testis using 26434-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26434-1-AP (SUNC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 26434-1-AP

  IP result of anti-SUNC1 (IP:26434-1-AP, 4ug; Detection:26434-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IF Staining of HepG2 using 26434-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SUNC1 antibody (26434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

26434-1-AP bindet in WB, IF/ICC, IP, CoIP, ELISA SUNC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUNC1 fusion protein Ag23780
• Vollständiger Name: Sad1 and UNC84 domain containing 1
• Beobachtetes Molekulargewicht: 37-41 kDa
• GenBank-Zugangsnummer: BC026189
• Gene symbol: SUNC1
• Gene ID (NCBI): 256979
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SUNC1 encodes SUN3 which is a member of SUN domain protein family, an evolutionary conserved family of inner nuclear membrane (INM) proteins that share a common C-terminal motif. SUN3 is mainly expressed in testis and is involved in sperm head formation.

Publikationen

Species	Application	Title
mouse	WB,CoIP	J Biol Chem; The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis.; Authors - Qian Gao
mouse	WB	Cell Mol Life Sci; CCDC28A deficiency causes sperm head defects, reduced sperm motility and male infertility in mice; Authors - Hongbin Zhou