SUPT16H Monoklonaler Antikörper

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SUPT16H Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E4C4

Kat-Nr. : 68243-1-PBS

Synonyme

FACT140, FACT 140 kDa subunit, Facilitates chromatin transcription complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, CDC68

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  WB analysis using 68243-1-Ig (same clone as 68243-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68243-1-Ig (SUPT16H antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68243-1-Ig (same clone as 68243-1-PBS)

  WB result of SUPT16H antibody (68243-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUPT16H transfected MCF-7 cells. This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 68243-1-Ig (same clone as 68243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68243-1-Ig (SUPT16H antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68243-1-Ig (same clone as 68243-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUPT16H antibody (68243-1-Ig, Clone: 1E4C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68243-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68243-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SUPT16H und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SUPT16H fusion protein Ag29459
• Vollständiger Name: suppressor of Ty 16 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 120 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: NM_007192
• Gene symbol: SUPT16H
• Gene ID (NCBI): 11198
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SUPT16H, also named as FACT140, FACTP140, SPT16 and CDC68, belongs to the peptidase M24 family and SPT16 subfamily. SUPT16H is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). It also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.