SUPT5H Polyklonaler Antikörper

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• KD/KO Validated

SUPT5H Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 16511-1-AP

Synonyme

SPT5, hSPT5, DSIF p160, DSIF large subunit, DRB sensitivity-inducing factor large subunit

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  WB analysis of HeLa using 16511-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16511-1-AP (SUPT5H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16511-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16511-1-AP (SUPT5H Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16511-1-AP

  IP result of anti-SUPT5H (IP:16511-1-AP, 4ug; Detection:16511-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of mouse testis using 16511-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16511-1-AP (SUPT5H antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16511-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16511-1-AP (SUPT5H antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16511-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUPT5H antibody (16511-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 16511-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUPT5H antibody (16511-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below

Produktinformation

16511-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SUPT5H und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUPT5H fusion protein Ag9728
• Vollständiger Name: suppressor of Ty 5 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 1087 aa, 121 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC024203
• Gene symbol: SUPT5H
• Gene ID (NCBI): 6829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SUPT5H, also named as DSIF p160, is a 1087 amino acid protein, which belongs to the SPT5 family. SUPT5H localizes in the nucleus and is ubiquitously expressed. SUPT5H is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H is modified through phosphorylation and methylated, so the molecular weight of SUPT5H might be 140-150 kDa.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpoint; Authors - Daniel Blears
human	WB	Mol Cell; SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.; Authors - Shibin Hu ; KD Validated
human	WB	Mol Cell; INTAC endonuclease and phosphatase modules differentially regulate transcription by RNA polymerase II; Authors - Shibin Hu
		Mol Cell; Three-step mechanism of promoter escape by RNA polymerase II; Authors - Yumeng Zhan
		Mol Cell; CDK7 kinase activity promotes RNA polymerase II promoter escape by facilitating initiation factor release; Authors - Taras Velychko
		Mol Cell; The PNUTS phosphatase complex controls transcription pause release; Authors - Jessica R Kelley