SURF1 Polyklonaler Antikörper

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• KD/KO Validated

SURF1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15379-1-PBS

Synonyme

SURF 1, SURF-1, surfeit 1, Surfeit locus protein 1

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  WB analysis using 15379-1-AP (same clone as 15379-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15379-1-AP (SURF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15379-1-AP (same clone as 15379-1-PBS)

  IP result of anti-SURF1 (IP:15379-1-AP, 4ug; Detection:15379-1-AP 1:2000) with HeLa cells lysate 1575 ug. This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 15379-1-AP (same clone as 15379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 15379-1-AP (same clone as 15379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15379-1-AP (same clone as 15379-1-PBS)

  Immunofluorescent analysis of HeLa cells using 15379-1-AP (SURF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15379-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SURF1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SURF1 fusion protein Ag3959
• Vollständiger Name: surfeit 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 30-31 kDa
• GenBank-Zugangsnummer: BC028314
• Gene symbol: SURF1
• Gene ID (NCBI): 6834
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.