SURF4 Polyklonaler Antikörper

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• KD/KO Validated

SURF4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 11599-1-AP

Synonyme

surfeit 4, SURF-4, SURF 4, ERV29

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• 

  WB analysis using 11599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11599-1-AP (SURF4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11599-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11599-1-AP (SURF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11599-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11599-1-AP (SURF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11599-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11599-1-AP (SURF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11599-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11599-1-AP (SURF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

11599-1-AP bindet in WB, IHC, ELISA SURF4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SURF4 fusion protein Ag2175
• Vollständiger Name: surfeit 4
• Berechnetes Molekulargewicht: 269 aa, 30 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC018741
• Gene symbol: SURF4
• Gene ID (NCBI): 6836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	J Transl Med; Identifying prognostic markers in spatially heterogeneous breast cancer microenvironment; Authors - Guohong Liu
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Inducible global knockout of surfeit locus protein 4 in adult mice results in hypolipidemia, intestinal lipid accumulation, liver injury, and increased mortality; Authors - Wei Chen ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fiona (Verified Customer) (10-19-2021)

Used for immunofluorescence on HeLa cells. Good, clear signal with minimal background at 1:500 without antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500