SURVIVIN Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

SURVIVIN Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B9H7

Kat-Nr. : 66495-1-PBS

Synonyme

BIRC5, IAP4, EPR 1, BIRC 5, Baculoviral IAP repeat-containing protein 5

---

• 

  WB analysis using 66495-1-Ig (same clone as 66495-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 66495-1-Ig (same clone as 66495-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66495-1-Ig (same clone as 66495-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66495-1-Ig (same clone as 66495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66495-1-Ig (same clone as 66495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66495-1-Ig (same clone as 66495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66495-1-Ig (same clone as 66495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66495-1-PBS bindet in WB, IHC, Indirect ELISA SURVIVIN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SURVIVIN fusion protein Ag20958
• Vollständiger Name: baculoviral IAP repeat-containing 5
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC008718
• Gene symbol: Survivin
• Gene ID (NCBI): 332
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.