SUSD2 Rekombinanter Antikörper

SUSD2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242328A8

Kat-Nr. : 84852-4-RR

Synonyme

242328A8, BK65A6.2, sushi domain containing 2

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  WB analysis of SK-BR-3 using 84852-4-RR

  SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 84852-4-RR (SUSD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 84852-4-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84852-4-RR (SUSD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of SK-BR-3 using 84852-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed SK-BR-3 cells using SUSD2 antibody (84852-4-RR, Clone: 242328A8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SK-BR-3 cells, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF/ICC: SK-BR-3 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84852-4-RR bindet in WB, IF/ICC, ELISA SUSD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SUSD2 fusion protein Eg2627
• Vollständiger Name: sushi domain containing 2
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 60 kDa, 90 kDa, 110 kDa
• GenBank-Zugangsnummer: NM_019601.3
• Gene symbol: SUSD2
• Gene ID (NCBI): 56241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sushi domain containing 2 (SUSD2) is a type I transmembrane protein that contains a somatomedin B, AMOP, von Willebrand factor type D and Sushi domains, which are frequently found in molecules associated with cell-cell and cell-matrix adhesion. SUSD2 is composed of 822 amino acids with a predicted molecular weight of 90.4 kDa (PMID: 31387209). Three specific SUSD2 bands were present, the glycosylated, full-length protein (~110 kDa), ~90 kDa band represents full-length SUSD2 with minimal glycosylation, and a strong ~60 kDa band, represents a cleaved product of SUSD2 at the GD-PH sequence in the endoplasmic reticulum (PMID:27775699, 32595828).