SUV39H1 Polyklonaler Antikörper

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• KD/KO Validated

SUV39H1 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10574-1-PBS

Synonyme

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  WB analysis using 10574-1-AP (same clone as 10574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10574-1-AP (same clone as 10574-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse small intestine using 10574-1-AP (same clone as 10574-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10574-1-AP (same clone as 10574-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10574-1-AP (same clone as 10574-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 10574-1-AP (same clone as 10574-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 10574-1-AP (same clone as 10574-1-PBS)

  IP result of anti-SUV39H1 (IP:10574-1-AP, 4ug; Detection:10574-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10574-1-PBS bindet in WB, IP, Indirect ELISA SUV39H1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUV39H1 fusion protein Ag0854
• Vollständiger Name: suppressor of variegation 3-9 homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC006238
• Gene symbol: SUV39H1
• Gene ID (NCBI): 6839
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone-lysine N-methyltransferase (SUV39H1), a nuclear protein, moves to the centromeres during mitosis and specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. It plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. SUV39H1 has 2 isoforms with molecular weights of 48 and 49 kDa.