SV2C Polyklonaler Antikörper

SV2C Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 21271-1-AP

Synonyme

KIAA1054, SV2C

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• 

  WB analysis of mouse brain using 21271-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21271-1-AP (SV2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 21271-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21271-1-AP (SV2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 21271-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 21271-1-AP (SV2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21271-1-AP

  IP result of anti-SV2C (IP:21271-1-AP, 4ug; Detection:21271-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of mouse cerebellum using 21271-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21271-1-AP (SV2C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 21271-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21271-1-AP (SV2C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, C6-Zellen, Maushautgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

21271-1-AP bindet in WB, IHC, IP, ELISA SV2C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SV2C fusion protein Ag15745
• Vollständiger Name: synaptic vesicle glycoprotein 2C
• Berechnetes Molekulargewicht: 727 aa, 82 kDa
• Beobachtetes Molekulargewicht: 80-95 kDa
• GenBank-Zugangsnummer: BC100826
• Gene symbol: SV2C
• Gene ID (NCBI): 22987
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Brain Res Bull; Pad2 Deletion Induces Anxiety‑like Behaviors and Memory Deficits in Mice; Authors - Xiaoqiang Lv

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (08-06-2024)

SV2C (in green) marked specifically some neurons (in red) in FFPE human brain tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

SV2C Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:21271-1-AP)