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SWAP70 Polyklonaler Antikörper

SWAP70 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31042-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using SWAP70 Polyclonal antibody (31042-1-AP)

    WB analysis using 31042-1-AP (same clone as 31042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31042-1-AP (SWAP70 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31042-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31042-1-PBS bindet in WB, Indirect ELISA SWAP70 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SWAP70 fusion protein Ag34855
Vollständiger Name SWAP-70 protein
Berechnetes Molekulargewicht 69 kDa
Beobachtetes Molekulargewicht65-70 kDa
GenBank-ZugangsnummerBC000616
Gene symbol SWAP70
Gene ID (NCBI) 23075
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SWAP70 (switching B cell complex subunit), also known as HSPC321. It is expected to be located in cell membrane, cytoplasm and nucleus, the protein is mainly expressed in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes, macrophages and mature B-cells (PMID: 10681448). The calculated molecular weight of SWAP70 is 69 kDa. SWAP-70 is a component of an enzyme complex that recombines Ig switch regions in vitro. It is reported that the cloning of the human cDNA and its B lymphocyte-specific expression. Although its sequence contains three nuclear localization signals, in small resting B cells, SWAP-70 is mainly found in the cytoplasm. On stimulation, SWAP-70 translocates to the nucleus. In activated, class-switching B cell cultures, it is associated with membrane IgG, but not IgM. The membrane Ig association requires a functional pleckstrin homology domain and is controlled by the C terminus. It is suggested that SWAP-70 is involved not only in nuclear events but also in signaling in B cell activation (PMID: 10681448).