SWAP70 Rekombinanter Antikörper

SWAP70 Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240641F5

Kat-Nr. : 83646-6-PBS

Synonyme

SWAP-70, SWAP 70 protein, SWAP 70, KIAA0640, HSPC321

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  WB analysis using 83646-6-RR (same clone as 83646-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83646-6-RR (SWAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83646-6-RR (same clone as 83646-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83646-6-RR (SWAP70 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83646-6-RR (same clone as 83646-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83646-6-RR (SWAP70 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

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  FC experiment of SK-N-SH using 83646-6-RR (same clone as 83646-6-PBS)

  1x10^6 SK-N-SH cells were intracellularly stained with 0.25 ug SWAP70 Recombinant antibody (83646-6-RR, Clone:240641F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83646-6-RR (same clone as 83646-6-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.2 ug SWAP70 Recombinant antibody (83646-6-RR, Clone:240641F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83646-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83646-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83646-6-RR against Human SWAP70 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83646-6-PBS bindet in WB, IHC, FC (Intra), ELISA SWAP70 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SWAP70 fusion protein Ag34855
• Vollständiger Name: SWAP-70 protein
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC000616
• Gene symbol: SWAP70
• Gene ID (NCBI): 23075
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SWAP70 (switching B cell complex subunit), also known as HSPC321. It is expected to be located in cell membrane, cytoplasm and nucleus, the protein is mainly expressed in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes, macrophages and mature B-cells (PMID: 10681448). The calculated molecular weight of SWAP70 is 69 kDa. SWAP-70 is a component of an enzyme complex that recombines Ig switch regions in vitro. It is reported that the cloning of the human cDNA and its B lymphocyte-specific expression. Although its sequence contains three nuclear localization signals, in small resting B cells, SWAP-70 is mainly found in the cytoplasm. On stimulation, SWAP-70 translocates to the nucleus. In activated, class-switching B cell cultures, it is associated with membrane IgG, but not IgM. The membrane Ig association requires a functional pleckstrin homology domain and is controlled by the C terminus. It is suggested that SWAP-70 is involved not only in nuclear events but also in signaling in B cell activation (PMID: 10681448).