SYAP1 Monoklonaler Antikörper

SYAP1 Monoklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E12A5

Kat-Nr. : 68410-1-Ig

Synonyme

DKFZp686K221, FLJ14495, FLJ44185, PRO3113, SYAP1, Synapse associated protein 1

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Galerie der Validierungsdaten

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  WB analysis using 68410-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68410-1-Ig (SYAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IP experiment of L02 using 68410-1-Ig

  IP result of anti-SYAP1(IP:68410-1-Ig, 4ug; Detection:68410-1-Ig 1:2000) with L02 cells lysate 3080 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, A549-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68410-1-Ig bindet in WB, IP, ELISA SYAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SYAP1 fusion protein Ag9626
• Vollständiger Name: synapse associated protein 1, SAP47 homolog (Drosophila)
• Berechnetes Molekulargewicht: 352 aa, 40 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC014657
• Gene symbol: SYAP1
• Gene ID (NCBI): 94056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.