SYF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SYF2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (3)

Kat-Nr. : 16958-1-AP

Synonyme

Pre-mRNA-splicing factor SYF2, Pre mRNA splicing factor SYF2, GCIPIP, CCNDBP1-interactor, CCNDBP1 interactor

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• 

  WB analysis of HEK-293 using 16958-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16958-1-AP (SYF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16958-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16958-1-AP (SYF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 16958-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 16958-1-AP (SYF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• RIP: See 1 publications below

Produktinformation

16958-1-AP bindet in WB, IF/ICC, RIP, ELISA SYF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SYF2 fusion protein Ag10656
• Vollständiger Name: SYF2 homolog, RNA splicing factor (S. cerevisiae)
• Berechnetes Molekulargewicht: 243 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC010862
• Gene symbol: SYF2
• Gene ID (NCBI): 25949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,RIP	Nucleic Acids Res; ZRANB2 and SYF2-mediated splicing programs converging on ECT2 are involved in breast cancer cell resistance to doxorubicin.; Authors - Iris Tanaka ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Moritz (Verified Customer) (09-24-2025)

Worked well for Syf2 detection in HEK293T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T

SYF2 Antibody Western Blot validation (1:3000 dilution) in HEK293T (Cat no:16958-1-AP)

FH

Aleksandr (Verified Customer) (07-19-2025)

A major band on Syf2 is detected. Antibody was incubated overnight at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

SYF2 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:16958-1-AP)

FH

Tsimafei (Verified Customer) (08-07-2024)

Antibody recognize a strong band of the correct size. Also two lower nolecular weight non-specific bands are visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

SYF2 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:16958-1-AP)