SYK Polyklonaler Antikörper

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• KD/KO Validated

SYK Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 22206-1-AP

Synonyme

EC:2.7.10.2, IMD82, p72-Syk, spleen tyrosine kinase, Tyrosine protein kinase SYK

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  WB analysis of HEK-293 using 22206-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22206-1-AP (SYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22206-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22206-1-AP (SYK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 22206-1-AP

  IP result of anti-SYK (IP:22206-1-AP, 4ug; Detection:22206-1-AP 1:500) with HEK-293 cells lysate 2700ug.

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  IHC staining of human spleen using 22206-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22206-1-AP (SYK Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 22206-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22206-1-AP (SYK Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Ramos using 22206-1-AP

  1x10^6 Ramos cells were intracellularly stained with 0.4 ug SYK Polyclonal antibody (22206-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

22206-1-AP bindet in WB, IHC, IP, ELISA SYK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SYK fusion protein Ag17526
• Vollständiger Name: spleen tyrosine kinase
• Berechnetes Molekulargewicht: 635 aa, 72 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC001645
• Gene symbol: SYK
• Gene ID (NCBI): 6850
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)

Publikationen

Species	Application	Title
human	WB	Virulence; Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK.; Authors - Xuelin Han
mouse	WB	Biochim Biophys Acta; Syk negatively regulates TLR4-mediated IFNβ and IL-10 production and promotes inflammatory responses in dendritic cells.; Authors - Hui Yin ; KO Validated