Symplekin Polyklonaler Antikörper

Symplekin Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 11519-1-AP

Synonyme

SPK, SYM, SYMPK, symplekin

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  WB analysis of K-562 using 11519-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11519-1-AP (Symplekin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11519-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11519-1-AP (Symplekin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 11519-1-AP

  IP result of anti-Symplekin (IP:11519-1-AP, 4ug; Detection:11519-1-AP 1:500) with K-562 cells lysate 3600ug.

• 

  IHC staining of human testis using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

11519-1-AP bindet in WB, IHC, IF, IP, ELISA Symplekin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Symplekin fusion protein Ag2081
• Vollständiger Name: symplekin
• Berechnetes Molekulargewicht: 1274 aa, 141 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC006567
• Gene symbol: Symplekin
• Gene ID (NCBI): 8189
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Front Cell Dev Biol; SYMPK Is Required for Meiosis and Involved in Alternative Splicing in Male Germ Cells; Authors - Rui Wu ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (09-12-2025)

Detects endogenous protein with high sensitivity in Western blot both in HeLa and Jurkat cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat, HeLa

Symplekin Antibody Western Blot validation (1:1000 dilution) in Jurkat, HeLa (Cat no:11519-1-AP)