SYN1-Specific Polyklonaler Antikörper

SYN1-Specific Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(66)

Rezensionen (2)

Kat-Nr. : 20258-1-AP

Synonyme

SYN1, Synapsin I, Brain protein 4.1, SYN1a, SYN1b

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  WB analysis of mouse brain using 20258-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20258-1-AP (SYN1-Specific Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 20258-1-AP

  IP result of anti-SYN1-Specific (IP:20258-1-AP, 4ug; Detection:20258-1-AP 1:3000) with rat brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 20258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20258-1-AP (SYN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20258-1-AP (SYN1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat eye using 20258-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using SYN1-Specific antibody (20258-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Ratten-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 53 publications below
• IHC: See 2 publications below
• IF: See 22 publications below

Produktinformation

20258-1-AP bindet in WB, IHC, IF-P, IP, ELISA SYN1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: synapsin I
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: NM_006950
• Gene symbol: SYN1
• Gene ID (NCBI): 6853
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYN1, also named Brain protein 4.1, belongs to the synapsin family. SYN1 is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders (XELBD).

Publikationen

Species	Application	Title
mouse	WB	Environ Health Perspect; Evaluation of Neurotoxicity in BALB/c Mice following Chronic Exposure to Polystyrene Microplastics; Authors - Haibo Jin
rat	WB	Neurobiol Stress; Low-dose S-ketamine exerts antidepressant-like effects via enhanced hippocampal synaptic plasticity in postpartum depression rats.; Authors - Zhuoyu Ren
human	WB,IF	Prog Neurobiol; Islet Amyloid Polypeptide Triggers α-synuclein Pathology in Parkinson's Disease; Authors - Lanxia Meng
mouse	WB	Brain Behav Immun; Hippocampal alpha-synuclein mediates depressive-like behaviors.; Authors - Tingfu Du
mouse	WB	Sci Total Environ; Manganese-induced α-synuclein overexpression promotes the accumulation of dysfunctional synaptic vesicles and hippocampal synaptotoxicity by suppressing Rab26-dependent autophagy in presynaptic neurons; Authors - Zhuo Ma
mouse	WB	ACS Chem Neurosci; Merazin Hydrate Produces Rapid Antidepressant Effects Depending on Activating mTOR Signaling by Upregulating Downstream Synaptic Proteins in the Hippocampus.; Authors - Chao Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical cells went trough ICC and the SYN1 antibody at the 1/50 dilution and MAP2 antibody at the 1/1000 dilution were used overnight at 4°C.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: Human primary cortical cells

SYN1-Specific Antibody Immunohistochemistry validation (1/50 dilution) in Human primary cortical cells (Cat no:20258-1-AP)

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain