SYN1-Specific Rekombinanter Antikörper

SYN1-Specific Rekombinant Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

242415H5

Kat-Nr. : 85360-1-RR

Synonyme

SYN1, Synapsin I, 242415H5, Brain protein 4.1, SYN1a

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  WB analysis using 85360-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85360-1-RR (SYN1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse retina using 85360-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse retina tissue using SYN1-Specific antibody (85360-1-RR, Clone: 242415H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: IMR-32 cells, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF-P: Maus-Retina-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85360-1-RR bindet in WB, IF-P, ELISA SYN1-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: synapsin I
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: NM_006950
• Gene symbol: SYN1
• Gene ID (NCBI): 6853
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYN1, also named Brain protein 4.1, belongs to the synapsin family. SYN1 is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders (XELBD).