SYNGAP1 Polyklonaler Antikörper

SYNGAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19739-1-PBS

Synonyme

Neuronal RasGAP, Ras GTPase activating protein SynGAP, Ras/Rap GTPase-activating protein SynGAP, RASA 1, Synaptic Ras GTPase-activating protein 1

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  WB analysis using 19739-1-AP (same clone as 19739-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19739-1-AP (SYNGAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19739-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 19739-1-AP (same clone as 19739-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19739-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 19739-1-AP (same clone as 19739-1-PBS)

  IP result of anti-SYNGAP1 (IP:19739-1-AP, 4ug; Detection:19739-1-AP 1:1500) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 19739-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19739-1-AP (same clone as 19739-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19739-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19739-1-AP (same clone as 19739-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19739-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 19739-1-AP (same clone as 19739-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using SYNGAP1 antibody (19739-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19739-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19739-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SYNGAP1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: synaptic Ras GTPase activating protein 1 homolog (rat)
• Berechnetes Molekulargewicht: 1343 aa, 148 kDa
• Beobachtetes Molekulargewicht: 148 kDa
• GenBank-Zugangsnummer: NM_006772
• Gene symbol: SYNGAP1
• Gene ID (NCBI): 8831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYNGAP1, also named as KIAA1938, is the major constituent of the PSD essential for postsynaptic signaling. It's an inhibitory regulator of the Ras-cAMP pathway. SYNGAP1 is a member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. SYNGAP1 regulates AMPAR-mediated miniature excitatory postsynaptic currents. SYNGAP1 may be involved in certain forms of brain injury, leading to long-term learning and memory deficits Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5).