SYNJ1 Polyklonaler Antikörper

SYNJ1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24677-1-PBS

Synonyme

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  WB analysis of mouse brain using 24677-1-AP (same clone as 24677-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24677-1-AP (same clone as 24677-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 24677-1-AP (same clone as 24677-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 24677-1-AP (same clone as 24677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 24677-1-AP (same clone as 24677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 24677-1-AP (same clone as 24677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 24677-1-AP (same clone as 24677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 24677-1-AP (same clone as 24677-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 24677-1-AP (SYNJ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24677-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SYNJ1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SYNJ1 fusion protein Ag20364
• Vollständiger Name: synaptojanin 1
• Berechnetes Molekulargewicht: 1612 aa, 178 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC136603
• Gene symbol: SYNJ1
• Gene ID (NCBI): 8867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptojanin 1, a polyphosphoinositide phosphatase, is expressed as two major alternatively spliced isoforms of 145 kDa (SJ145) and 170 kDa (SJ170), which are thought to have pleiotropic roles in endocytosis, signaling, and actin function. SJ145 is highly enriched in nerve terminals where it participates in clathrin-dependent synaptic vesicle recycling. SJ170, which differs from SJ145 by the presence of a carboxy-terminal extension, is the predominant isoform in developing neurons and is expressed in a variety of tissues (PMID:10801423).