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SYNJ2BP Polyklonaler Antikörper
SYNJ2BP Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 15666-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Mausnierengewebe, A549-Zellen, Mauslungengewebe, Rattennierengewebe |
| Erfolgreiche IP | Mauslungengewebe |
| Erfolgreiche Detektion in IHC | Mauslebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, MCF-7-Zellen |
| Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:200-1:800 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 11 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Produktinformation
15666-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA SYNJ2BP und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | SYNJ2BP fusion protein Ag8130 |
| Vollständiger Name | synaptojanin 2 binding protein |
| Berechnetes Molekulargewicht | 145 aa, 16 kDa |
| Beobachtetes Molekulargewicht | 16 kDa |
| GenBank-Zugangsnummer | BC007704 |
| Gene symbol | SYNJ2BP |
| Gene ID (NCBI) | 55333 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. SYNJ2BP is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. SYNJ2BP may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. A double band of size 15.9 kDa can be detected in Western blot (PMID: 23284606).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for SYNJ2BP antibody 15666-1-AP | Protokoll herunterladen |
| IHC protocol for SYNJ2BP antibody 15666-1-AP | Protokoll herunterladenl |
| IF protocol for SYNJ2BP antibody 15666-1-AP | Protokoll herunterladen |
| IP protocol for SYNJ2BP antibody 15666-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Neuron Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy. | ||
Sci Adv ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution | ||
Cell Rep Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology. | ||
Oncotarget SYNJ2BP promotes the degradation of PTEN through the lysosome-pathway and enhances breast tumor metastasis via PI3K/AKT/SNAI1 signaling.
| ||
J Cell Sci Mitochondrial antiviral-signalling protein is a client of the BAG6 protein quality control complex. |







