SYNJ2BP Polyklonaler Antikörper

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• KD/KO Validated

SYNJ2BP Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 15666-1-AP

Synonyme

ARIP2, OMP25, Synaptojanin-2-binding protein

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  WB analysis using 15666-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15666-1-AP (SYNJ2BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15666-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15666-1-AP (SYNJ2BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 15666-1-AP

  IP result of anti-SYNJ2BP (IP:15666-1-AP, 3ug; Detection:15666-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of mouse liver using 15666-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15666-1-AP (SYNJ2BP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15666-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15666-1-AP (SYNJ2BP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15666-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SYNJ2BP antibody (15666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15666-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SYNJ2BP antibody (15666-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of HepG2 using 15666-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SYNJ2BP (15666-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, A549-Zellen, Mauslungengewebe, Rattennierengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

15666-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA SYNJ2BP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SYNJ2BP fusion protein Ag8130
• Vollständiger Name: synaptojanin 2 binding protein
• Berechnetes Molekulargewicht: 145 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC007704
• Gene symbol: SYNJ2BP
• Gene ID (NCBI): 55333
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. SYNJ2BP is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. SYNJ2BP may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. A double band of size 15.9 kDa can be detected in Western blot (PMID: 23284606).

Publikationen

Species	Application	Title
human	WB	Science; The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.; Authors - Michael J McKenna
	IF	Neuron; Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy.; Authors - Angelika B Harbauer
	WB	Sci Adv; ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution; Authors - Michael J McKenna
human	WB	Cell Rep; Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.; Authors - Fenglei Jian
mouse,human	WB,IP,IHC	Oncotarget; SYNJ2BP promotes the degradation of PTEN through the lysosome-pathway and enhances breast tumor metastasis via PI3K/AKT/SNAI1 signaling.; Authors - Miao Wang ; KD Validated
human	WB	J Cell Sci; Mitochondrial antiviral-signalling protein is a client of the BAG6 protein quality control complex.; Authors - Peristera Roboti