SYNJ2BP Rekombinanter Antikörper

SYNJ2BP Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250205D7

Kat-Nr. : 85870-4-RR

Synonyme

ARIP2, OMP25, Synaptojanin-2-binding protein

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  WB analysis of rat kidney using 85870-4-RR

  Rat kidney tissue lysates were subjected to SDS PAGE followed by western blot with 85870-4-RR (ARIP2; SYNJ2BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85870-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85870-4-RR (ARIP2; SYNJ2BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85870-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARIP2; SYNJ2BP antibody (85870-4-RR, Clone: 250205D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85870-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85870-4-RR against Human SYNJ2BP were performed. The affinity constant is 0.68 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Herzgewebe, Rattennierengewebe, SGC-7901-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85870-4-RR bindet in WB, IF/ICC, ELISA SYNJ2BP und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SYNJ2BP fusion protein Ag8130
• Vollständiger Name: synaptojanin 2 binding protein
• Berechnetes Molekulargewicht: 145 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC007704
• Gene symbol: SYNJ2BP
• Gene ID (NCBI): 55333
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. SYNJ2BP is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. SYNJ2BP may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. A double band of size 15.9 kDa can be detected in Western blot (PMID: 23284606).