SYNM Polyklonaler Antikörper

SYNM Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20735-1-AP

Synonyme

Desmuslin, DMN, KIAA0353, SYN, Synemin, SYNM

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Galerie der Validierungsdaten

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  WB analysis using 20735-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20735-1-AP (SYNM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. SYNM gene methylation in T-47D cells causes gene silencing.

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  WB analysis of human skeletal muscle using 20735-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20735-1-AP (SYNM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human bladder using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20735-1-AP

  Immunofluorescent analysis of HeLa cells using 20735-1-AP (SYNM antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Blasengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20735-1-AP bindet in WB, IHC, IF, ELISA SYNM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: synemin, intermediate filament protein
• Berechnetes Molekulargewicht: 173 kDa
• Beobachtetes Molekulargewicht: 170-180 kDa
• GenBank-Zugangsnummer: NM_015286
• Gene symbol: SYNM
• Gene ID (NCBI): 23336
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYNM, also named as DMN, KIAA0353 and SYN, belongs to the intermediate filament family. SYNM is a Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells. The antibody can't recognize isoform 2 or 3 of SYNM.

Publikationen

Species	Application	Title
human	IHC	J Ovarian Res; A risk model of gene signatures for predicting platinum response and survival in ovarian cancer.; Authors - Siyu Chen
human	IF	bioRxiv; Tropism of SARS-CoV-2 for Developing Human Cortical Astrocytes.; Authors - Madeline G Andrews