SYNPO Polyklonaler Antikörper

SYNPO Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(70)

Kat-Nr. : 21064-1-AP

Synonyme

Synaptopodin, snaptopodin, KIAA1029

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  WB analysis of mouse brain using 21064-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21064-1-AP (Synaptopodin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21064-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21064-1-AP (Synaptopodin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 21064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21064-1-AP (Synaptopodin antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21064-1-AP (Synaptopodin antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of rat brain using 21064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Synaptopodin antibody (21064-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse kidney using 21064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SYNPO antibody (21064-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe, Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 33 publications below
• IHC: See 8 publications below
• IF: See 42 publications below

Produktinformation

21064-1-AP bindet in WB, IHC, IF-P, ELISA SYNPO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SYNPO fusion protein Ag15089
• Vollständiger Name: synaptopodin
• Berechnetes Molekulargewicht: 929 aa, 99 kDa
• Beobachtetes Molekulargewicht: 99-115 kDa
• GenBank-Zugangsnummer: BC146665
• Gene symbol: SYNPO
• Gene ID (NCBI): 11346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

Publikationen

Species	Application	Title
mouse	IF	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang
human	IF	Biofabrication; A glomerulus chip with spherically twisted cell-laden hollow fibers as glomerular capillary tufts; Authors - Zhilin Dai
human	IF	Theranostics; Renal tubular Bim mediates the tubule-podocyte crosstalk via NFAT2 to induce podocyte cytoskeletal dysfunction.; Authors - Chunmei Xu
human,mouse	IF	Diabetes; Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.; Authors - Hua Qu
mouse	IF	Free Radic Biol Med; P2X7R/AKT/mTOR signaling mediates high glucose-induced decrease in podocyte autophagy; Authors - Cheng Qian
mouse	WB,IF	Mol Ther Nucleic Acids; Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy.; Authors - Ziyang Wang