SYNPO2L Rekombinanter Antikörper

SYNPO2L Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240523F12

Kat-Nr. : 83532-3-PBS

Synonyme

240523F12, Synaptopodin 2 like protein, Synaptopodin 2-like protein

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  IF Staining of HepG2 using 83532-3-RR (same clone as 83532-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SYNPO2L antibody (83532-3-RR, Clone: 240523F12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83532-3-PBS in a different storage buffer formulation.

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  FC experiment of Caco-2 using 83532-3-RR (same clone as 83532-3-PBS)

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SYNPO2L Recombinant antibody (83532-3-RR, Clone:240523F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83532-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00513-1

  Cytometric bead array standard curve of MP00513-1, SYNPO2L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83532-3-PBS. Detection antibody: 83532-1-PBS. Standard: Ag34027. Range: 1.56-100 ng/mL.

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  Cytometric bead array standard curve of MP00513-2

  Cytometric bead array standard curve of MP00513-2, SYNPO2L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83532-3-PBS. Detection antibody: 83532-4-PBS. Standard: Ag34027. Range: 1.56-100 ng/mL.

• 

  Cytometric bead array standard curve of MP00513-3

  Cytometric bead array standard curve of MP00513-3, SYNPO2L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83532-3-PBS. Detection antibody: 83532-2-PBS. Standard: Ag34027. Range: 3.13-100 ng/mL.

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  Affinity and Kinetic Characterization of 83532-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83532-3-RR against Human SYNPO2L were performed. The affinity constant is 80.5 pM.

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Geprüfte Anwendungen

Produktinformation

83532-3-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA SYNPO2L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SYNPO2L fusion protein Ag34027
• Vollständiger Name: synaptopodin 2-like
• Berechnetes Molekulargewicht: 977 aa, 102 kDa
• GenBank-Zugangsnummer: BC142635
• Gene symbol: SYNPO2L
• Gene ID (NCBI): 79933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.