Synaptoporin Polyklonaler Antikörper

Synaptoporin Polyklonal Antikörper für WB, IHC, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 14143-1-AP

Synonyme

SYNPR, SPO

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  WB analysis using 14143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14143-1-AP (Synaptoporin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14143-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14143-1-AP (Synaptoporin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 14143-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14143-1-AP (Synaptoporin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14143-1-AP

  IP result of anti-Synaptoporin (IP:14143-1-AP, 4ug; Detection:14143-1-AP 1:2000) with mouse brain tissue lysate 1720 ug.

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  IHC staining of mouse brain using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14143-1-AP (Synaptoporin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14143-1-AP (Synaptoporin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

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  IHC staining of human gliomas using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

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  IHC staining of human colon using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

• 

  IHC staining of human colon using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

• 

  IHC staining of human brain using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14143-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of rat brain using 14143-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using Synaptoporin antibody (14143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, humanes Kolongewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-Fro: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

14143-1-AP bindet in WB, IHC, IF-Fro, IP, ELISA Synaptoporin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Synaptoporin fusion protein Ag5307
• Vollständiger Name: synaptoporin
• Berechnetes Molekulargewicht: 265 aa, 29 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC022518
• Gene symbol: Synaptoporin
• Gene ID (NCBI): 132204
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Biomedicines; Combined DiI and Antibody Labeling Reveals Complex Dysgenesis of Hippocampal Dendritic Spines in a Mouse Model of Fragile X Syndrome; Authors - Luisa Speranza
mouse	IF	CNS Neurosci Ther; Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation; Authors - Limin Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cajsa (Verified Customer) (10-07-2024)

Great antibody!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Myenteric plexus of adult mouse intestine