Synaptophysin Polyklonaler Antikörper

Synaptophysin Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(256)

Kat-Nr. : 17785-1-AP

Synonyme

SYP, MRX96, SYN



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, Maus-Cerebellum-Gewebe, PC-12-Zellen, Rattenhirngewebe, Ratten-Cerebellum-Gewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHCMaus-Pankreasgewebe, Gangliomgewebe, humanes Hirngewebe, humanes Pankreasgewebe, humanes Hypophysenadenom-Gewebe, Maushirngewebe, Neuroblastomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaus-Pankreasgewebe, humanes Gliomgewebe, humanes Lungenkarzinomgewebe, Maushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:20000-1:100000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17785-1-AP bindet in WB, IHC, IF-P, IP, ELISA Synaptophysin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, hamster, Hund, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Synaptophysin fusion protein Ag11781
Vollständiger Name synaptophysin
Berechnetes Molekulargewicht 313 aa, 34 kDa
Beobachtetes Molekulargewicht 38-40 kDa
GenBank-ZugangsnummerBC064550
Gene symbol Synaptophysin
Gene ID (NCBI) 6855
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for the identification of neuroendocrine cells and neoplasms. Synaptic proteins including synaptophysin are reduced in brain tissue from patients with Alzheimer's disease.(PMID: 3010302; 21658579)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Synaptophysin antibody 17785-1-APProtokoll herunterladen
IHC protocol for Synaptophysin antibody 17785-1-APProtokoll herunterladenl
IF protocol for Synaptophysin antibody 17785-1-APProtokoll herunterladen
IP protocol for Synaptophysin antibody 17785-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
rat,mouseWB,IF

Nat Genet

Identification of TMEM230 mutations in familial Parkinson's disease.

Authors - Han-Xiang Deng
mouseWB,IF

Nat Neurosci

Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage

Authors - Yao Wei
mouseIF

Mol Cell

Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury.

Authors - Zhengxin Ying
mouseWB

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
mouseIF

Neuron

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Authors - Nathalie Escande-Beillard

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Alexandra (Verified Customer) (06-11-2023)

Hoechst-blue Green-Tubulin III (neuronal marker) Orange (Synaptophysin)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: P19 derived neurons
Synaptophysin Antibody Immunofluorescence validation (1:300 dilution) in P19 derived neurons (Cat no:17785-1-AP)
FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical neurons was labelled with 1/1000 of the Synaptophysin Polyclonal antibody. The labelling was strong and very good. Blue (Bisbenzimide) Green (Synaptophysin)

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human primary cortical neurons
Synaptophysin Antibody Immunohistochemistry validation (1/1000 dilution) in Human primary cortical neurons (Cat no:17785-1-AP)