Synaptotagmin-1 Rekombinanter Antikörper

Synaptotagmin-1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240589F6

Kat-Nr. : 83637-4-PBS

Synonyme

Synaptotagmin 1, SYT1, SYT, synaptotagmin I, SVP65

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  WB analysis using 83637-4-RR (same clone as 83637-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83637-4-RR (Synaptotagmin-1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83637-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83637-4-RR (same clone as 83637-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83637-4-RR (Synaptotagmin-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83637-4-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 83637-4-RR (same clone as 83637-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Synaptotagmin-1 antibody (83637-4-RR, Clone: 240589F6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83637-4-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 83637-4-RR (same clone as 83637-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Synaptotagmin-1 antibody (83637-4-RR, Clone: 240589F6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83637-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83637-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83637-4-RR against Human Synaptotagmin-1 were performed. The affinity constant is 20.5 nM.

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Geprüfte Anwendungen

Produktinformation

83637-4-PBS bindet in WB, IHC, IF/ICC, ELISA Synaptotagmin-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Synaptotagmin-1 fusion protein Ag5970
• Vollständiger Name: synaptotagmin I
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC058917
• Gene symbol: Synaptotagmin-1
• Gene ID (NCBI): 6857
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.