SYT17 Polyklonaler Antikörper

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• KD/KO Validated

SYT17 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 15413-1-AP

Synonyme

synaptotagminXVII, Synaptotagmin-17, Synaptotagmin17, Synaptotagmin XVII, Synaptotagmin 17

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  WB analysis of human kidney using 15413-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15413-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15413-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15413-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15413-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15413-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15413-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 15413-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15413-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15413-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15413-1-AP

  IP result of anti-SYT17 (IP:15413-1-AP, 4ug; Detection:15413-1-AP 1:800) with mouse brain tissue lysate 6000ug.

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  IHC staining of human brain using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 15413-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SYT17 antibody (15413-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, A375-Zellen, humanes Hirngewebe, humanes Hodengewebe, Maushirngewebe, Mauslungengewebe, PC-3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15413-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SYT17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SYT17 fusion protein Ag7691
• Vollständiger Name: synaptotagmin XVII
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa
• GenBank-Zugangsnummer: BC004518
• Gene symbol: SYT17
• Gene ID (NCBI): 51760
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYT17, or synaptotagmin 17, is a protein that belongs to the synaptotagmin family, which is known for its role in regulating exocytosis at the plasma membrane. Reports find high levels of expression in hippocampus, particularly in pyramidal cell layers and in cultured hippocampal neurons. SYT17 is thus a multifunctional regulator of intracellular protein trafficking in excitatory hippocampal neurons, modulating both neural development and synaptic physiology. (PMID: 31387992, PMID: 36332665)

Publikationen

Species	Application	Title
human	IHC,IF	Int Immunol; Increased urinary exosomal SYT17 levels in chronic active antibody-mediated rejection after kidney transplantation via the IL-6 amplifier.; Authors - Yusuke Takada ; KD Validated