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Sam50 Monoklonaler Antikörper

Sam50 Monoklonal Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H6H9

Kat-Nr. : 67425-1-PBS

Synonyme

SAMM50, 2H6H9, CGI 51, CGI-51, OMP85

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Sam50 Monoclonal antibody (67425-1-Ig)

    IF Staining of HepG2 using 67425-1-Ig (same clone as 67425-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Sam50 Monoclonal antibody (67425-1-Ig)

    IF Staining of HeLa using 67425-1-Ig (same clone as 67425-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Sam50 Monoclonal antibody (67425-1-Ig)

    IF Staining of HepG2 using 67425-1-Ig (same clone as 67425-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67425-1-PBS bindet in IF/ICC, ELISA Sam50 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Sam50 fusion protein Ag15155
Vollständiger Name sorting and assembly machinery component 50 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 469 aa, 52 kDa
Beobachtetes Molekulargewicht 52 kDa
GenBank-ZugangsnummerBC007830
Gene symbol Sam50
Gene ID (NCBI) 25813
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).