Sam68 Polyklonaler Antikörper

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• KD/KO Validated

Sam68 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10222-1-PBS

Synonyme

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  WB analysis using 10222-1-AP (same clone as 10222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10222-1-AP (Sam68 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

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  WB analysis using 10222-1-AP (same clone as 10222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10222-1-AP (Sam68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10222-1-AP (same clone as 10222-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10222-1-AP (same clone as 10222-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 10222-1-AP (same clone as 10222-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10222-1-AP (same clone as 10222-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10222-1-AP (same clone as 10222-1-PBS)

  IP result of anti-Sam68 (IP:10222-1-AP, 4ug; Detection:10222-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10222-1-AP (same clone as 10222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10222-1-AP (KHDRBS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10222-1-AP (same clone as 10222-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10222-1-AP (Sam68 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10222-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA Sam68 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Sam68 fusion protein Ag0235
• Vollständiger Name: KH domain containing, RNA binding, signal transduction associated 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC000717
• Gene symbol: Sam68
• Gene ID (NCBI): 10657
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KHDRBS1, also named as SAM68, p62and p68, belongs to the KHDRBS family. It is recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and INS receptors. Once phosphorylated, KHDRBS1 functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. KHDRBS1 also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. KHDRBS1 has three isoforms with calculated MW of 44-48kd. For isoform with a calculated MW of 48.2 kD, it migrated as a 68kD protein on SDS-PAGE gels[PMID: 10564820]. This ia a rabbit polyclonal antibody raised against residues near the N terminus of human KHDRBS1.

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