Securin Polyklonaler Antikörper

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Securin Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18040-1-PBS

Synonyme

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  WB analysis using 18040-1-AP (same clone as 18040-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18040-1-AP (Securin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 18040-1-AP (same clone as 18040-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 18040-1-AP (Securin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18040-1-AP (same clone as 18040-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Securin / PTTG1 antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 18040-1-AP (same clone as 18040-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Securin antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18040-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Securin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Securin fusion protein Ag12670
• Vollständiger Name: pituitary tumor-transforming 1
• Berechnetes Molekulargewicht: 202 aa, 22 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC101834
• Gene symbol: Securin
• Gene ID (NCBI): 9232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.