Selenoprotein P Polyklonaler Antikörper

Selenoprotein P Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30249-1-PBS

Synonyme

SEPP1, SELENOP

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  WB analysis of human plasma using 30249-1-AP (same clone as 30249-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 30249-1-AP (Selenoprotein P antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30249-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 30249-1-AP (same clone as 30249-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30249-1-AP (Selenoprotein P antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30249-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 30249-1-AP (same clone as 30249-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Selenoprotein P antibody (30249-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30249-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 30249-1-AP (same clone as 30249-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Selenoprotein P Polyclonal antibody (30249-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 30249-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30249-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Selenoprotein P und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Selenoprotein P fusion protein Ag32952
• Vollständiger Name: selenoprotein P, plasma, 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: NM_001085486.2
• Gene symbol: Selenoprotein P
• Gene ID (NCBI): 6414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Selenoprotein P, also known as SEPP1, is expressed in the liver and heart and secreted into the plasma(PMID: 8421687). Selenoprotein P is implicated as an extracellular antioxidant involved in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2) and provides selenium to tissues such as brain and testis. In humans, it exists in plasma as two isoforms of approximately 50 and 60 kDa(PMID:19453253). Selenoprotein P is a glycosylated protein and deglycosylation of SEPP1 results in 45 kDa and 35 kDa isoforms(PMID: 25298198).