Selenoprotein P Rekombinanter Antikörper

Selenoprotein P Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242510E7

Kat-Nr. : 85017-4-RR

Synonyme

SEPP1, 242510E7

---

• 

  WB analysis using 85017-4-RR

  Rat plasma were subjected to SDS PAGE followed by western blot with 85017-4-RR (Selenoprotein P antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human milk using 85017-4-RR

  human milk were subjected to SDS PAGE followed by western blot with 85017-4-RR (Selenoprotein P antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85017-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85017-4-RR against Human Selenoprotein P were performed. The affinity constant is 0.587 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human milk

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85017-4-RR bindet in WB, ELISA Selenoprotein P und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Selenoprotein P fusion protein Ag32952
• Vollständiger Name: selenoprotein P, plasma, 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 45-60 kDa
• GenBank-Zugangsnummer: NM_001085486.2
• Gene symbol: Selenoprotein P
• Gene ID (NCBI): 6414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Selenoprotein P, also known as SEPP1, is expressed in the liver and heart and secreted into the plasma(PMID: 8421687). Selenoprotein P is implicated as an extracellular antioxidant involved in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2) and provides selenium to tissues such as brain and testis. In humans, it exists in plasma as two isoforms of approximately 50 and 60 kDa(PMID:19453253). Selenoprotein P is a glycosylated protein and deglycosylation of SEPP1 results in 45 kDa and 35 kDa isoforms(PMID: 25298198).