P-Selectin/CD62P Rekombinanter Antikörper

P-Selectin/CD62P Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240766D3

Kat-Nr. : 83947-5-PBS

Synonyme

P-selectin, CD62P, 240766D3, PADGEM, P selectin

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  WB analysis of human peripheral blood leukocyte using 83947-5-RR (same clone as 83947-5-PBS)

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 83947-5-RR (same clone as 83947-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 83947-5-RR (same clone as 83947-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 83947-5-RR (same clone as 83947-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83947-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83947-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83947-5-RR against Mouse P-Selectin/CD62P were performed. The affinity constant is 7.97 nM.

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Geprüfte Anwendungen

Produktinformation

83947-5-PBS bindet in WB, IHC, IF-P, ELISA P-Selectin/CD62P und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: selectin, platelet
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_011347.2
• Gene symbol: Selp
• Gene ID (NCBI): 20344
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.