Septin 5 Polyklonaler Antikörper

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• KD/KO Validated

Septin 5 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11631-1-PBS

Synonyme

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  WB analysis of Jurkat using 11631-1-AP (same clone as 11631-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11631-1-AP (same clone as 11631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 11631-1-AP (same clone as 11631-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11631-1-AP (same clone as 11631-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 11631-1-AP (same clone as 11631-1-PBS)

  IP result of anti-Septin 5 (IP:11631-1-AP, 3ug; Detection:11631-1-AP 1:300) with mouse brain tissue lysate 1000ug. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11631-1-AP (same clone as 11631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11631-1-AP (same clone as 11631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11631-1-PBS bindet in WB, IHC, IP, Indirect ELISA Septin 5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Septin 5 fusion protein Ag2205
• Vollständiger Name: septin 5
• Berechnetes Molekulargewicht: 369 aa, 43 kDa
• Beobachtetes Molekulargewicht: 40-43 kDa
• GenBank-Zugangsnummer: BC025261
• Gene symbol: Septin 5
• Gene ID (NCBI): 5413
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.